Precise & Protective

Archive for February, 2015

Key steps to prevent pipetting errors

It is very important to avoid errors during any lab-work or research process. Here are some steps required to prevent pipetting errors during lab-work:-

Pipette tips

                 Micropipette Tips

  • You should clean and check your pipette properly after each use because a contamination-free pipette ensures accuracy. Make it a daily routine. Also, check for any damage prior to beginning work.
  • It is extremely essential to have your pipette serviced after every six months (on an average). The servicing of the pipette includes the complete disassembling of the pipette for re-calibration and replacement of broken parts.
  • Before using the pipettes, you should have a decent knowledge of proper pipetting techniques. Make sure you follow some simple techniques while using a pipette:
  • Start practicing a smooth and slow motion
  • Pre-wet the pipette tip before you start the process
  • Hold the pipette vertically when drawing in liquid and at a 45 degree angle when dispensing in liquid
  • Immerse the tip slightly into the liquid during the aspiration process
  • Touch off the pipette on the sidewall of the container
  • It is very important to use a pipette with a volume selection closest to the volume required for the aspiration or dispensing process. For example, if you need to dispense 15 microliters, a 20 microliter pipette would be an ideal choice.
  • The environmental conditions should be taken into account. You should consider the consistency of temperature, pressure, humidity, etc. before you start using pipettes for any research work. It is important because an inconsistent environment will affect the accuracy of your results as liquids are susceptible to all types of environmental changes. Also, it is safe to allow your pipette to adjust according to the environmental conditions.
  • Always try and keep your pipette safe like never hold your pipette when you’re not using it, never put it down on the lab bench, always keep it on a stand, always store it in an upright position e.tc.
  • It is better to use a new tip every time or whenever you test a new liquid. A tip that is continually used for different tests and substances is susceptible to cross contamination.

These few and simple tips can help you a lot during your research work. Remember, prevention is always better than cure.

Abdos Micro-pipette Tips: Universal and compatible with various other brands of pipettes

Serological Pipettes

             Pasteur Pipette

  • Abdos Tips are manufactured in state of art precision-moulded facility using high purity virgin polypropylene.
  • Micro Tips are DNase, RNase and Pyrogen free.
  • Designed for use in a wide variety of pipetting applications and are compatible with different types of u-Pipettes.
  • Clean and the hydrophobic surface ensures low retention, increased accuracy and reproducibility.
Variable Premium Pipette

          Fixed Premium Pipette

  • More transparency for clear visualization in molecular biology work.
  • High clarity and finer tip orifice makes the tip more suitable for molecular biology and precise applications.
  • Perfect airtight grip on pipette by inner radial rings for accurate pipetting.
  • The orifice is precisely centered for directional accuracy.
  • Can be autoclaved

For more information on code, capacity or compatibility of the Abdos micro-pipettes, log on to http://www.abdoslabware.com/micro-tips.html

How to measure volume using a Measuring Cylinder

A measuring cylinder is laboratory equipment, used to measure the volume of a liquid. Also, popular as graduated cylinder or mixing cylinder, the measuring cylinder provides more accurate and precise measurement than other laboratory flasks and beakers.

plastic measuring cylinder

plastic measuring cylinder

The measuring cylinders come in various sizes ranging from 10 ml to 1,000 ml. The readings are taken after measuring the lowest portion of the meniscus.

What is meniscus?

As per the information available through various sources, the meniscus is the lowest portion of the convex dip of the liquid as it settles in the measuring cylinder.

What is the reason of meniscus formation?

The formation of meniscus takes place due to the attraction of water molecules within the walls of the measuring cylinder. It is based on the principle that states- “Adhesive forces are stronger than cohesive forces”.

Steps involved in measuring or calculating the volume of a measuring cylinder

In order to calculate the volume with the help of a measuring cylinder, you will have to find the height (h), and the radius (r) of the cylinder and put it into a simple formula- V = hπr2. The steps are given below:

  • First, find the radius of the circular base of the measuring cylinder. If you know the diameter of the circle then you can divide it by 2 or if you know the circumference, then you have to divide it by 2π.
  • Second, calculate the area of the circular base. The formula for calculating the area of a circle will serve the purpose here, A = πr2.
  • Third, find the height of the cylinder. You can use a scale or a ruler to measure the height. The height is basically the distance between the edges of the two bases.
  • Fourth, after finding the area and the height, you have to multiply the area of the circular base by the height of the cylinder as the formula for measuring volume is V = hπr2.

Note:

The volume is always represented in cubic units because it is the measure of a three-dimensional space.

The π is about the equivalent of 22/7.

Some tips to remember during the measurement

  • Make sure that you have taken accurate measurements during the volume measurement process.
  • If you are not sure of your own calculations then go for a calculator. This will make your work easy and less time-consuming.
  • “Practice makes a man perfect”- this phrase works here too. Make up a few problems and practice it before you get down to do the process in real.
  • Memorise the necessary formulas involved so that during the process, you don’t have to struggle for it.

Abdos range of measuring cylinders

As a leading name in plastic lab-ware products, Abdos bring forth an extensive assortment of general laboratory products for daily experiments in a research laboratory. We design and manufacture these products at our moulding unit using high purity and specialized resins. We guarantee and certify the accuracy of our volumetric products thereby reassuring our researchers’ confidence in our products.

Our Mission

  • To expand the capacity at all our existing plants.
  • In order to cater the need of scientific research, setting up of more factories across the globe.
  • Promoting & generate the brand awareness by incorporating various Marketing activities and entering into a Digital Medium.

Abdos offers a wide range of measuring cylinders namely:-

Measuring Cylinder

Measuring Cylinder

  • Measuring Cylinders Tall Shape, PP Class “B”
  • Measuring Cylinders Tall Shape, TPX Class “B”
  • Measuring Cylinder, TPX Class “A”

For detailed information on the same, please visit http://www.abdoslabware.com/measuring-cylinders-pp.html

Magnetic Stirrers are better than Motorised Stirrers

A magnetic stirrer is a device that employs a rotating magnetic field to cause a stir bar immersed in a liquid to spin very quickly, thus stirring it. The rotating field can be created either by setting some stationary electromagnets or by a rotating magnet, placed beneath the vessel with the liquid.

Working principle of Magnetic Stirrer

The magnetic stirrers work on the principle of using an external magnetic field for mixing the solutions. The external magnetic field rotates a small magnetic bar that has been placed in the desired mixture. Unlike the conventional process of stirring or mixing liquids where the action is externally generated and usually mixing blades are used, magnetic stirring is accomplished by a coated magnet immersed in the sample container. The configuration of the immersed magnet can be that of a bar, oval, pellet or egg. The configuration of the magnet reportedly depends on the size and configuration of the sample container.

History of Magnetic Stirrer

The magnetic stirrer is also known as magnetic mixer and it is invented by Arthur Rosinger of Newark, New Jersey, USA. He obtained the US Patent 2,350,534, titled Magnetic Stirrer on 6 June 1944 that included a brief description of his invention.

The first multi-point magnetic stirrer was developed and patented by Salvador Bonet of SBS Company in 1977. He also introduced the practice of noting the denomination of stirring power in “liters of water”, which is a market standard today.

Advantages of Magnetic Stirrer

  • It minimizes the risks of contamination over conventional stirring or mixing systems since it only uses a stir bar or coated immersed magnets (also called ‘fleas’) that are chemically inert. These fleas can be easily cleaned and disinfected as compared to the more laborious cleaning and disinfecting steps involved in cleaning and disinfectant processes of mixing tools with complex configurations.
  • Also, they don’t require any kind of lubricants for the mixing process. The lubricants generally could contaminate the reaction vessel and the product as well.
  • It is critical for consistent and reproducible mixing i.e. it can mix the solutions over long time scales. For example: A protein dialysis requires multi-hour or overnight sample mixing. Also, protein dialysis is extremely sensitive to bacterial contamination.
  • Due to the flexible properties, they can also be used inside hermetically closed vessels or systems.
Magnetic Stirrer

WAVEMAX – Orbital Shaker

Why is a magnetic stirrer better than a motorized stirrer for lab purposes?

Magnetic stirrers are the most preferred devices for mixing liquids in chemistry and biological laboratories. It is far better than motorized stirrers because of following properties:-

  • Magnetic stirrers are quieter
  • They are more efficient
  • They don’t have any moving external parts so no fear of breaking or wearing out
  • They can be easily cleaned and sterilized
  • They don’t require lubricants so no fear of contamination during the process

Whereas, one may find these above mentioned difficulties while using motorized stirrers.

Abdos Labware offers magnetic stirrer: Swirl Top

Laboratory Hot Plate

A renowned name in Labware products, Abdos Labtech Pvt Ltd. offers SwirlTop magnetic stirrer with compatible and efficient features:-

  • Swirl Top unit feature an extremely durable, chemical resistant white ceramic work surface.
  • Swirl Top has advanced microprocessor control with convenient turn knobs that allow quick, precise adjustment and maintains accurate temperature and speed.
  • Swirl Top is compatible with a wide variety of popular glass beakers, flasks, bottles and vessels.
  • E11215 comes complete with support rod for mounting thermometers and temperature probes.

For a detailed information about the voltage, frequency, dimension, weight e.tc, please visit http://www.abdoslabware.com/wavemax-rocking-shaker.html

For a wide range of other Labware products and equipment, log on to http://www.abdoslabware.com/

We as team want to serve the scientific fraternity through our quality product and services. Maintaining the quality assurance, Abdos always look forward to create more-n-more plastic Labware products that make the lab work easier and efficient.

Centrifugation: A widely applied and crucial research technique

In the field of biochemistry, cellular and molecular biology, and even in medicine, centrifugation is one of the most significant and widely applied research techniques. In technical terms, it is a process which involves the use of the centrifugal force for the sedimentation of heterogeneous mixtures with a centrifuge. In layman terms, it is nothing but a process to separate components of a complex mixture or to separate two immiscible liquids by using a centrifuge. This process is an integral part of industry and laboratory settings.

Centrifugation

                   Centrifugation

If we talk about the field of proteomics, centrifugation plays a vital role in the fundamental and necessary process of isolating proteins. It is a known fact that centrifugation is probably the most commonly used and preferred method for separating out all the non-protein material.

Centrifugation Process

              Centrifugation Process

This process has other uses such as:-

Centrifugation Methods

            Centrifugation Method

  • It is used to remove cells or other suspended particles from their surrounding milieu on either a batch or a continuous-flow basis. It also, separates a cell type different from another one.
  • The process isolates viruses and macromolecules, including DNA, RNA, proteins, and lipids or establishes physical parameters of these particles from their usual observed behavior.
  • Centrifugation separates the various sub-cellular organelles including nuclei, mitochondria, chloroplasts, Golgi bodies, lysosomes, peroxisomes, glyoxysomes, plasma membranes, endoplasmic reticulum, polysomic, and ribosomal subunits from the dispersed tissue.

How the separation of components achieved in centrifugation process?

Centrifugation

The separation of the components during centrifugation is achieved by spinning a vessel containing material at a high speed. The imposed centrifugal force pushes heavier materials to the outside of the vessel (in some cases to settle down).

Centrifugation Methods

Centrifugation Methods

Centrifuge Tubes: In the whole process, the most recurring term is the centrifuge tubes. It is a laboratory device that is used for the separation of fluids, gas or liquid, based on density. It works on the principle that when the required samples are spun at a high speed, the resulting force causes the particles to separate based on their density. For example, those particles having a high density will settle down and those with less density will float.

Centrifuge Tubes

              Centrifuge Tubes

This highly useful apparatus is found in most of the laboratories starting from academic to clinical to research laboratories. The device is extremely useful for purification of cells, sub-cellular organelles, viruses, proteins and nucleic acids.

Also, these tubes are available in different types depending upon the rotor design or the intent if use. From the large floor variety to the micro-centrifuge, there are many varieties available for the researcher.

Abdos’ range of Centrifuge Ware

Abdos is a well-known global brand in manufacturing plastic lab-ware products. We started our plastic laboratory division and its operations, back in the year 2009 envisaging a clear vision of delivering quality products that will transform the research world into a better and hassle-free one.

Our broad range of Centrifuge Ware is employed in a variety of centrifugation methods and assay investigations in laboratories. We takes pride in molding these fine lab-products from unadulterated and virgin resins that yield low extractability and leaching while using them.

The products that comprise the Centrifuge Ware are listed below

1)         Micro Centrifuge Tubes, PP

2)         Micro Pestles, PP

3)         Centrifuge Tube Conical Bottom, PP/HDPE

4)         Self Standing Centrifuge Tube

5)         Centrifuge Tube Conical Bottom in Polystyrene

6)         Racked Centrifuge Tube Conical Bottom

7)         Rack for Micro Centrifuge Tubes, RPP

8)         Rack for Micro Centrifuge Tubes, PC

9)         Centrifuge Tube Box, PP

10)      Swirl-01 Micro Centrifuge

Microcentrifuges

     Micro Centrifuge Tubes

Centrifuge Tube Conical Bottom

Centrifuge Tube Conical Bottom

Rack for Micro Centrifuge Tubes

Rack for Micro Centrifuge Tubes

These products act as consumable items in the laboratories while displaying their distinctive characteristics and fine quality during laboratory research processes.