Precise & Protective

Archive for the ‘Laboratory Liquid Handling Products’ Category

Key steps to prevent pipetting errors

It is very important to avoid errors during any lab-work or research process. Here are some steps required to prevent pipetting errors during lab-work:-

Pipette tips

                 Micropipette Tips

  • You should clean and check your pipette properly after each use because a contamination-free pipette ensures accuracy. Make it a daily routine. Also, check for any damage prior to beginning work.
  • It is extremely essential to have your pipette serviced after every six months (on an average). The servicing of the pipette includes the complete disassembling of the pipette for re-calibration and replacement of broken parts.
  • Before using the pipettes, you should have a decent knowledge of proper pipetting techniques. Make sure you follow some simple techniques while using a pipette:
  • Start practicing a smooth and slow motion
  • Pre-wet the pipette tip before you start the process
  • Hold the pipette vertically when drawing in liquid and at a 45 degree angle when dispensing in liquid
  • Immerse the tip slightly into the liquid during the aspiration process
  • Touch off the pipette on the sidewall of the container
  • It is very important to use a pipette with a volume selection closest to the volume required for the aspiration or dispensing process. For example, if you need to dispense 15 microliters, a 20 microliter pipette would be an ideal choice.
  • The environmental conditions should be taken into account. You should consider the consistency of temperature, pressure, humidity, etc. before you start using pipettes for any research work. It is important because an inconsistent environment will affect the accuracy of your results as liquids are susceptible to all types of environmental changes. Also, it is safe to allow your pipette to adjust according to the environmental conditions.
  • Always try and keep your pipette safe like never hold your pipette when you’re not using it, never put it down on the lab bench, always keep it on a stand, always store it in an upright position e.tc.
  • It is better to use a new tip every time or whenever you test a new liquid. A tip that is continually used for different tests and substances is susceptible to cross contamination.

These few and simple tips can help you a lot during your research work. Remember, prevention is always better than cure.

Abdos Micro-pipette Tips: Universal and compatible with various other brands of pipettes

Serological Pipettes

             Pasteur Pipette

  • Abdos Tips are manufactured in state of art precision-moulded facility using high purity virgin polypropylene.
  • Micro Tips are DNase, RNase and Pyrogen free.
  • Designed for use in a wide variety of pipetting applications and are compatible with different types of u-Pipettes.
  • Clean and the hydrophobic surface ensures low retention, increased accuracy and reproducibility.
Variable Premium Pipette

          Fixed Premium Pipette

  • More transparency for clear visualization in molecular biology work.
  • High clarity and finer tip orifice makes the tip more suitable for molecular biology and precise applications.
  • Perfect airtight grip on pipette by inner radial rings for accurate pipetting.
  • The orifice is precisely centered for directional accuracy.
  • Can be autoclaved

For more information on code, capacity or compatibility of the Abdos micro-pipettes, log on to http://www.abdoslabware.com/micro-tips.html

How to measure volume using a Measuring Cylinder

A measuring cylinder is laboratory equipment, used to measure the volume of a liquid. Also, popular as graduated cylinder or mixing cylinder, the measuring cylinder provides more accurate and precise measurement than other laboratory flasks and beakers.

plastic measuring cylinder

plastic measuring cylinder

The measuring cylinders come in various sizes ranging from 10 ml to 1,000 ml. The readings are taken after measuring the lowest portion of the meniscus.

What is meniscus?

As per the information available through various sources, the meniscus is the lowest portion of the convex dip of the liquid as it settles in the measuring cylinder.

What is the reason of meniscus formation?

The formation of meniscus takes place due to the attraction of water molecules within the walls of the measuring cylinder. It is based on the principle that states- “Adhesive forces are stronger than cohesive forces”.

Steps involved in measuring or calculating the volume of a measuring cylinder

In order to calculate the volume with the help of a measuring cylinder, you will have to find the height (h), and the radius (r) of the cylinder and put it into a simple formula- V = hπr2. The steps are given below:

  • First, find the radius of the circular base of the measuring cylinder. If you know the diameter of the circle then you can divide it by 2 or if you know the circumference, then you have to divide it by 2π.
  • Second, calculate the area of the circular base. The formula for calculating the area of a circle will serve the purpose here, A = πr2.
  • Third, find the height of the cylinder. You can use a scale or a ruler to measure the height. The height is basically the distance between the edges of the two bases.
  • Fourth, after finding the area and the height, you have to multiply the area of the circular base by the height of the cylinder as the formula for measuring volume is V = hπr2.

Note:

The volume is always represented in cubic units because it is the measure of a three-dimensional space.

The π is about the equivalent of 22/7.

Some tips to remember during the measurement

  • Make sure that you have taken accurate measurements during the volume measurement process.
  • If you are not sure of your own calculations then go for a calculator. This will make your work easy and less time-consuming.
  • “Practice makes a man perfect”- this phrase works here too. Make up a few problems and practice it before you get down to do the process in real.
  • Memorise the necessary formulas involved so that during the process, you don’t have to struggle for it.

Abdos range of measuring cylinders

As a leading name in plastic lab-ware products, Abdos bring forth an extensive assortment of general laboratory products for daily experiments in a research laboratory. We design and manufacture these products at our moulding unit using high purity and specialized resins. We guarantee and certify the accuracy of our volumetric products thereby reassuring our researchers’ confidence in our products.

Our Mission

  • To expand the capacity at all our existing plants.
  • In order to cater the need of scientific research, setting up of more factories across the globe.
  • Promoting & generate the brand awareness by incorporating various Marketing activities and entering into a Digital Medium.

Abdos offers a wide range of measuring cylinders namely:-

Measuring Cylinder

Measuring Cylinder

  • Measuring Cylinders Tall Shape, PP Class “B”
  • Measuring Cylinders Tall Shape, TPX Class “B”
  • Measuring Cylinder, TPX Class “A”

For detailed information on the same, please visit http://www.abdoslabware.com/measuring-cylinders-pp.html

Centrifugation: A widely applied and crucial research technique

In the field of biochemistry, cellular and molecular biology, and even in medicine, centrifugation is one of the most significant and widely applied research techniques. In technical terms, it is a process which involves the use of the centrifugal force for the sedimentation of heterogeneous mixtures with a centrifuge. In layman terms, it is nothing but a process to separate components of a complex mixture or to separate two immiscible liquids by using a centrifuge. This process is an integral part of industry and laboratory settings.

Centrifugation

                   Centrifugation

If we talk about the field of proteomics, centrifugation plays a vital role in the fundamental and necessary process of isolating proteins. It is a known fact that centrifugation is probably the most commonly used and preferred method for separating out all the non-protein material.

Centrifugation Process

              Centrifugation Process

This process has other uses such as:-

Centrifugation Methods

            Centrifugation Method

  • It is used to remove cells or other suspended particles from their surrounding milieu on either a batch or a continuous-flow basis. It also, separates a cell type different from another one.
  • The process isolates viruses and macromolecules, including DNA, RNA, proteins, and lipids or establishes physical parameters of these particles from their usual observed behavior.
  • Centrifugation separates the various sub-cellular organelles including nuclei, mitochondria, chloroplasts, Golgi bodies, lysosomes, peroxisomes, glyoxysomes, plasma membranes, endoplasmic reticulum, polysomic, and ribosomal subunits from the dispersed tissue.

How the separation of components achieved in centrifugation process?

Centrifugation

The separation of the components during centrifugation is achieved by spinning a vessel containing material at a high speed. The imposed centrifugal force pushes heavier materials to the outside of the vessel (in some cases to settle down).

Centrifugation Methods

Centrifugation Methods

Centrifuge Tubes: In the whole process, the most recurring term is the centrifuge tubes. It is a laboratory device that is used for the separation of fluids, gas or liquid, based on density. It works on the principle that when the required samples are spun at a high speed, the resulting force causes the particles to separate based on their density. For example, those particles having a high density will settle down and those with less density will float.

Centrifuge Tubes

              Centrifuge Tubes

This highly useful apparatus is found in most of the laboratories starting from academic to clinical to research laboratories. The device is extremely useful for purification of cells, sub-cellular organelles, viruses, proteins and nucleic acids.

Also, these tubes are available in different types depending upon the rotor design or the intent if use. From the large floor variety to the micro-centrifuge, there are many varieties available for the researcher.

Abdos’ range of Centrifuge Ware

Abdos is a well-known global brand in manufacturing plastic lab-ware products. We started our plastic laboratory division and its operations, back in the year 2009 envisaging a clear vision of delivering quality products that will transform the research world into a better and hassle-free one.

Our broad range of Centrifuge Ware is employed in a variety of centrifugation methods and assay investigations in laboratories. We takes pride in molding these fine lab-products from unadulterated and virgin resins that yield low extractability and leaching while using them.

The products that comprise the Centrifuge Ware are listed below

1)         Micro Centrifuge Tubes, PP

2)         Micro Pestles, PP

3)         Centrifuge Tube Conical Bottom, PP/HDPE

4)         Self Standing Centrifuge Tube

5)         Centrifuge Tube Conical Bottom in Polystyrene

6)         Racked Centrifuge Tube Conical Bottom

7)         Rack for Micro Centrifuge Tubes, RPP

8)         Rack for Micro Centrifuge Tubes, PC

9)         Centrifuge Tube Box, PP

10)      Swirl-01 Micro Centrifuge

Microcentrifuges

     Micro Centrifuge Tubes

Centrifuge Tube Conical Bottom

Centrifuge Tube Conical Bottom

Rack for Micro Centrifuge Tubes

Rack for Micro Centrifuge Tubes

These products act as consumable items in the laboratories while displaying their distinctive characteristics and fine quality during laboratory research processes.

How to use the Micropipette Tips

Pipettes are a common sight in any chemistry, biology or a physics laboratory. Popularly known as pipette, pipettor, chemical dropper or a pipet, it is a common laboratory tool to transfer a measured volume of liquid. They are available in different designs for different purposes.

High Quality Micropipette Tips

Micropipette Tips Sizes

Micropipettes are those dispensing between 1 and 1000 μl and in contrast, macropipettes dispense greater volumes. Depending upon the sizes, capacity and style, pipettes is of following types:

•    Air displacement micropipettes
•    Positive displacement pipette
•    Volumetric pipettes
•    Graduated pipettes
•    Pasteur pipette
•    Transfer pipettes
•    Van Slyke pipette
•    Ostwald–Folin pipette
•    Glass micropipette
•    Extremely low volume pipettes
•    Microfluidic pipette

Micro Pipettes

Laboratory Micropipettes

But before using a pipette, it is extremely necessary that you master their use in order to be successful in the lab experiments.

Parts of Micropipette

a)    Plunger button
b)    Tip ejector button
c)    Volume adjustment dial
d)    Digital volume indicator
e)    Shaft
f)    Attachment point for a disposable tip

Sizes of micropipette

Basically, micropipettes come in three different sizes of varying capacity. They are P20, P200 and P1000. The sizes are noted on the top of the plunger button (as mentioned earlier, part a)

Pipette Tips

You should always remember that liquids are never drawn directly into the shaft of the pipette. For this, a disposable plastic tips are attached to the shaft and for adjusting the volume of the liquid, there is a black volume adjustment dial near the top of the micropipette.

There are two sizes of tips. The larger blue tips are used for the P1000 and the smaller clear tips are used for the P20 and P200. You should also take care of not contaminating the tips, for example: – close the box of the tips (racked in), after the use so that airborne contamination can be prevented.

How to insert a tip

Micropipette tips uses

Micropipette tips function

Before inserting a tip, you should keep some essential points in mind as given below:-
•    Select the correct size tips.
•    Open the box without touching the tips with your hands.
•    Insert the micropipette shaft into the tip and press down firmly. This will attach the tip to the shaft.
•    Remove the micropipette, with the tip attached.
•    Close the box without touching the tips with your hands
•    Remember, it is better to handle something carefully rather than doing it like a defaulter.