Precise & Protective

Archive for the ‘Labware Products’ Category

Key steps to prevent pipetting errors

It is very important to avoid errors during any lab-work or research process. Here are some steps required to prevent pipetting errors during lab-work:-

Pipette tips

                 Micropipette Tips

  • You should clean and check your pipette properly after each use because a contamination-free pipette ensures accuracy. Make it a daily routine. Also, check for any damage prior to beginning work.
  • It is extremely essential to have your pipette serviced after every six months (on an average). The servicing of the pipette includes the complete disassembling of the pipette for re-calibration and replacement of broken parts.
  • Before using the pipettes, you should have a decent knowledge of proper pipetting techniques. Make sure you follow some simple techniques while using a pipette:
  • Start practicing a smooth and slow motion
  • Pre-wet the pipette tip before you start the process
  • Hold the pipette vertically when drawing in liquid and at a 45 degree angle when dispensing in liquid
  • Immerse the tip slightly into the liquid during the aspiration process
  • Touch off the pipette on the sidewall of the container
  • It is very important to use a pipette with a volume selection closest to the volume required for the aspiration or dispensing process. For example, if you need to dispense 15 microliters, a 20 microliter pipette would be an ideal choice.
  • The environmental conditions should be taken into account. You should consider the consistency of temperature, pressure, humidity, etc. before you start using pipettes for any research work. It is important because an inconsistent environment will affect the accuracy of your results as liquids are susceptible to all types of environmental changes. Also, it is safe to allow your pipette to adjust according to the environmental conditions.
  • Always try and keep your pipette safe like never hold your pipette when you’re not using it, never put it down on the lab bench, always keep it on a stand, always store it in an upright position e.tc.
  • It is better to use a new tip every time or whenever you test a new liquid. A tip that is continually used for different tests and substances is susceptible to cross contamination.

These few and simple tips can help you a lot during your research work. Remember, prevention is always better than cure.

Abdos Micro-pipette Tips: Universal and compatible with various other brands of pipettes

Serological Pipettes

             Pasteur Pipette

  • Abdos Tips are manufactured in state of art precision-moulded facility using high purity virgin polypropylene.
  • Micro Tips are DNase, RNase and Pyrogen free.
  • Designed for use in a wide variety of pipetting applications and are compatible with different types of u-Pipettes.
  • Clean and the hydrophobic surface ensures low retention, increased accuracy and reproducibility.
Variable Premium Pipette

          Fixed Premium Pipette

  • More transparency for clear visualization in molecular biology work.
  • High clarity and finer tip orifice makes the tip more suitable for molecular biology and precise applications.
  • Perfect airtight grip on pipette by inner radial rings for accurate pipetting.
  • The orifice is precisely centered for directional accuracy.
  • Can be autoclaved

For more information on code, capacity or compatibility of the Abdos micro-pipettes, log on to http://www.abdoslabware.com/micro-tips.html

How to measure volume using a Measuring Cylinder

A measuring cylinder is laboratory equipment, used to measure the volume of a liquid. Also, popular as graduated cylinder or mixing cylinder, the measuring cylinder provides more accurate and precise measurement than other laboratory flasks and beakers.

plastic measuring cylinder

plastic measuring cylinder

The measuring cylinders come in various sizes ranging from 10 ml to 1,000 ml. The readings are taken after measuring the lowest portion of the meniscus.

What is meniscus?

As per the information available through various sources, the meniscus is the lowest portion of the convex dip of the liquid as it settles in the measuring cylinder.

What is the reason of meniscus formation?

The formation of meniscus takes place due to the attraction of water molecules within the walls of the measuring cylinder. It is based on the principle that states- “Adhesive forces are stronger than cohesive forces”.

Steps involved in measuring or calculating the volume of a measuring cylinder

In order to calculate the volume with the help of a measuring cylinder, you will have to find the height (h), and the radius (r) of the cylinder and put it into a simple formula- V = hπr2. The steps are given below:

  • First, find the radius of the circular base of the measuring cylinder. If you know the diameter of the circle then you can divide it by 2 or if you know the circumference, then you have to divide it by 2π.
  • Second, calculate the area of the circular base. The formula for calculating the area of a circle will serve the purpose here, A = πr2.
  • Third, find the height of the cylinder. You can use a scale or a ruler to measure the height. The height is basically the distance between the edges of the two bases.
  • Fourth, after finding the area and the height, you have to multiply the area of the circular base by the height of the cylinder as the formula for measuring volume is V = hπr2.

Note:

The volume is always represented in cubic units because it is the measure of a three-dimensional space.

The π is about the equivalent of 22/7.

Some tips to remember during the measurement

  • Make sure that you have taken accurate measurements during the volume measurement process.
  • If you are not sure of your own calculations then go for a calculator. This will make your work easy and less time-consuming.
  • “Practice makes a man perfect”- this phrase works here too. Make up a few problems and practice it before you get down to do the process in real.
  • Memorise the necessary formulas involved so that during the process, you don’t have to struggle for it.

Abdos range of measuring cylinders

As a leading name in plastic lab-ware products, Abdos bring forth an extensive assortment of general laboratory products for daily experiments in a research laboratory. We design and manufacture these products at our moulding unit using high purity and specialized resins. We guarantee and certify the accuracy of our volumetric products thereby reassuring our researchers’ confidence in our products.

Our Mission

  • To expand the capacity at all our existing plants.
  • In order to cater the need of scientific research, setting up of more factories across the globe.
  • Promoting & generate the brand awareness by incorporating various Marketing activities and entering into a Digital Medium.

Abdos offers a wide range of measuring cylinders namely:-

Measuring Cylinder

Measuring Cylinder

  • Measuring Cylinders Tall Shape, PP Class “B”
  • Measuring Cylinders Tall Shape, TPX Class “B”
  • Measuring Cylinder, TPX Class “A”

For detailed information on the same, please visit http://www.abdoslabware.com/measuring-cylinders-pp.html

Magnetic Stirrers are better than Motorised Stirrers

A magnetic stirrer is a device that employs a rotating magnetic field to cause a stir bar immersed in a liquid to spin very quickly, thus stirring it. The rotating field can be created either by setting some stationary electromagnets or by a rotating magnet, placed beneath the vessel with the liquid.

Working principle of Magnetic Stirrer

The magnetic stirrers work on the principle of using an external magnetic field for mixing the solutions. The external magnetic field rotates a small magnetic bar that has been placed in the desired mixture. Unlike the conventional process of stirring or mixing liquids where the action is externally generated and usually mixing blades are used, magnetic stirring is accomplished by a coated magnet immersed in the sample container. The configuration of the immersed magnet can be that of a bar, oval, pellet or egg. The configuration of the magnet reportedly depends on the size and configuration of the sample container.

History of Magnetic Stirrer

The magnetic stirrer is also known as magnetic mixer and it is invented by Arthur Rosinger of Newark, New Jersey, USA. He obtained the US Patent 2,350,534, titled Magnetic Stirrer on 6 June 1944 that included a brief description of his invention.

The first multi-point magnetic stirrer was developed and patented by Salvador Bonet of SBS Company in 1977. He also introduced the practice of noting the denomination of stirring power in “liters of water”, which is a market standard today.

Advantages of Magnetic Stirrer

  • It minimizes the risks of contamination over conventional stirring or mixing systems since it only uses a stir bar or coated immersed magnets (also called ‘fleas’) that are chemically inert. These fleas can be easily cleaned and disinfected as compared to the more laborious cleaning and disinfecting steps involved in cleaning and disinfectant processes of mixing tools with complex configurations.
  • Also, they don’t require any kind of lubricants for the mixing process. The lubricants generally could contaminate the reaction vessel and the product as well.
  • It is critical for consistent and reproducible mixing i.e. it can mix the solutions over long time scales. For example: A protein dialysis requires multi-hour or overnight sample mixing. Also, protein dialysis is extremely sensitive to bacterial contamination.
  • Due to the flexible properties, they can also be used inside hermetically closed vessels or systems.
Magnetic Stirrer

WAVEMAX – Orbital Shaker

Why is a magnetic stirrer better than a motorized stirrer for lab purposes?

Magnetic stirrers are the most preferred devices for mixing liquids in chemistry and biological laboratories. It is far better than motorized stirrers because of following properties:-

  • Magnetic stirrers are quieter
  • They are more efficient
  • They don’t have any moving external parts so no fear of breaking or wearing out
  • They can be easily cleaned and sterilized
  • They don’t require lubricants so no fear of contamination during the process

Whereas, one may find these above mentioned difficulties while using motorized stirrers.

Abdos Labware offers magnetic stirrer: Swirl Top

Laboratory Hot Plate

A renowned name in Labware products, Abdos Labtech Pvt Ltd. offers SwirlTop magnetic stirrer with compatible and efficient features:-

  • Swirl Top unit feature an extremely durable, chemical resistant white ceramic work surface.
  • Swirl Top has advanced microprocessor control with convenient turn knobs that allow quick, precise adjustment and maintains accurate temperature and speed.
  • Swirl Top is compatible with a wide variety of popular glass beakers, flasks, bottles and vessels.
  • E11215 comes complete with support rod for mounting thermometers and temperature probes.

For a detailed information about the voltage, frequency, dimension, weight e.tc, please visit http://www.abdoslabware.com/wavemax-rocking-shaker.html

For a wide range of other Labware products and equipment, log on to http://www.abdoslabware.com/

We as team want to serve the scientific fraternity through our quality product and services. Maintaining the quality assurance, Abdos always look forward to create more-n-more plastic Labware products that make the lab work easier and efficient.

How to Operate the Centrifuge Tubes: Guidelines

The centrifuge tubes are cylinder shaped calibrated glass containers commonly used for the analysis and separation of various materials in the laboratory (centrifugation procedures). They needed to be fit into the centrifuge slots for the experiments.

microcentrifuge tubes

Centrifuge Tubes

These are used to test solid-liquid or immiscible liquid samples by the lab technicians. First, they deposit various substances into the centrifuge tubes and then they secure them into the centrifuge within the slots. For the rotation, the lab technicians spin the tubes at proper speeds until the denser particles in each substance gather at the point farthest from the axis and vice-versa.

Materials used

A wide range of laboratory centrifuge tubes are available in plastic, copolymers, polypropylene and polystyrene.

Sizes

It ranges from micro-centrifuge tubes with caps to larger tubes capable of 50,000 grams in refrigerated centrifuges.

Types

There are different types of centrifuge tubes widely used for different purposes such as:-

Micro Centrifuge Tubes

Microcentrifuge Tubes with Snap Cap

  • Conical Bottom Tubes
  • Microcentrifuge Tubes- Snap Cap
  • Microcentrifuge Tubes- Screw Cap and so on

How to use a centrifuge to avoid mishaps

There are few key guidelines for operating a centrifuge tube in order to prevent damage to the centrifuge and possible serious injuries to the lab doctors or technicians:-

  • Always work on an even surface, don’t use the centrifuge on an uneven surface or a slanted one. The surface should be properly leveled and firm.
  • It is safe to balance the centrifuge tubes in the rotor, say for- if you want to run a centrifuge tube with 20 mL of liquid, it is smart to put another tube with 20 mL of water in the opposing hole on the rotor. And if the liquid has a varying density than water, then the best possible way is to balance the tubes by mass not volume.
  • Never ever, open the lid while the rotor is moving. It can be really dangerous at times. Although, there are many centrifuges that come with a “safety shutoff” if the lid is opened but it only stops the power of the rotor. The rotor will still spin due to its own inertia and thus it is better to be careful at the first place.
  • While the rotation of the centrifuge tubes, a little vibration is normal but excessive wobbling can mean danger. Always, double check whether the tubes are correctly balanced or not. Do not take the risk to continue running a centrifuge that is shaking or wobbling while the rotor is spinning.
  • If you are in a laboratory, it is always preferable to wear a face shield or safety glasses. And if you are working near a centrifuge then it is a must.
  • Do not touch, bump or move the centrifuge while the rotor is still spinning.
  • It is better to clean the rotor and centrifuge well after each use to avoid further disruptions.

How to use the Micropipette Tips

Pipettes are a common sight in any chemistry, biology or a physics laboratory. Popularly known as pipette, pipettor, chemical dropper or a pipet, it is a common laboratory tool to transfer a measured volume of liquid. They are available in different designs for different purposes.

High Quality Micropipette Tips

Micropipette Tips Sizes

Micropipettes are those dispensing between 1 and 1000 μl and in contrast, macropipettes dispense greater volumes. Depending upon the sizes, capacity and style, pipettes is of following types:

•    Air displacement micropipettes
•    Positive displacement pipette
•    Volumetric pipettes
•    Graduated pipettes
•    Pasteur pipette
•    Transfer pipettes
•    Van Slyke pipette
•    Ostwald–Folin pipette
•    Glass micropipette
•    Extremely low volume pipettes
•    Microfluidic pipette

Micro Pipettes

Laboratory Micropipettes

But before using a pipette, it is extremely necessary that you master their use in order to be successful in the lab experiments.

Parts of Micropipette

a)    Plunger button
b)    Tip ejector button
c)    Volume adjustment dial
d)    Digital volume indicator
e)    Shaft
f)    Attachment point for a disposable tip

Sizes of micropipette

Basically, micropipettes come in three different sizes of varying capacity. They are P20, P200 and P1000. The sizes are noted on the top of the plunger button (as mentioned earlier, part a)

Pipette Tips

You should always remember that liquids are never drawn directly into the shaft of the pipette. For this, a disposable plastic tips are attached to the shaft and for adjusting the volume of the liquid, there is a black volume adjustment dial near the top of the micropipette.

There are two sizes of tips. The larger blue tips are used for the P1000 and the smaller clear tips are used for the P20 and P200. You should also take care of not contaminating the tips, for example: – close the box of the tips (racked in), after the use so that airborne contamination can be prevented.

How to insert a tip

Micropipette tips uses

Micropipette tips function

Before inserting a tip, you should keep some essential points in mind as given below:-
•    Select the correct size tips.
•    Open the box without touching the tips with your hands.
•    Insert the micropipette shaft into the tip and press down firmly. This will attach the tip to the shaft.
•    Remove the micropipette, with the tip attached.
•    Close the box without touching the tips with your hands
•    Remember, it is better to handle something carefully rather than doing it like a defaulter.

Hot Plates- a friendly lab-ware

Hot plates are a common sight in many kitchens but their uses and advantages go beyond that!

A hot-plate (http://en.wikipedia.org/wiki/Hot_plate) is nothing but a portable self-contained tabletop small appliance which has two or more electric heating elements for heating. They also come with gas burners.

As the name suggests, these hot plates can be used as a standalone appliance. In many households, these are commonly used as a substitute for burners either from an oven or a stove. So next time if a kitchen stove is not or convenient for use, the hot plates can serve your purpose. You can indeed use them for cooking as they are extremely portable that is a great advantage during house parties. You can use them for sauté work or desserts on a table side or a buffet.

Magnetic Stirrer

Laboratory Hot Plate

For laboratorial settings and practices, these hot plates are usually used for heating glassware and the content in it. Some of them also contain a magnetic stirrer that automatically stirs the heated liquid in the glassware.

These are extremely useful in lab works because baths can be hazards if they spill, overheat or ignite. The main advantage of the hot plates is the cost. They are available at a very low cost in the market which allows them to be the most used appliance in this developing world. Those who have a limited budget, please do not worry, the most considered appropriate technology is here- hot plate. It is extremely pocket friendly and comes within the budget.

Working of a hot plate

It provides heating by using a disposable butane fuel cartridge. Usually after sparking an igniter, the hot plates will burn for 1.5 hours approx.

Advantages

  • It is possible and easy to heat stuff with disposable gas
  • It is available in larger sizes as compared to the induction cookers

Disadvantages

  • Check for the surfaces as they can get very hot at times
  • The generated heat takes time to adjust

Safety comes first

It is a known fact that safety is always good. You should keep some safety tips in mind while using a hot plate:-

  • Before heating content in a bath, check for the glassware’s heat resistance, cracks. Also, do remember that do not put any glassware on the plate directly. Apart from this, make sure that the surface of the hotplate is always larger than the vessel being heated.
  • Add boiling stones while bringing the liquids to boil. This will help in facilitating the process.
  • Do not condense any content/ material in a vessel until and unless it is completely dry. Even a single drop of moisture can eventually crack your vessel.
  • For heating liquids, a smart idea would be to set the temperature at medium or medium high setting.
  • Do not heat a metal plate on a hot plate as this can damage the appliance and often poses a shock risk.
  • Always use tongs or any rubber coated, heat resistant gripping devices while removing any object from a hot plate. This goes for pouring hot liquids too.
  • It is advisable not to store any flammable or volatile materials in the vicinity of a hot plate. Also, it is best to chuck out an old and worn-out hot plate for flammable materials.
  • Before using a hot plate, check carefully for corrosions in order to avoid any spark hazard.
  • Last but not the least, always remembers to turn it off after using.

Taking Care of your hot plate

  • A general cleaning way for your hot plate is to wipe the exterior surfaces with a piece of cloth dampened slightly. You can use a mild soap solution.
  • Do not use a vinegar solution for cleaning as it is just a myth.
  • Always keep the hot plate in cool and dry place. Try to avoid it from moisture as to keep it away from corrosion and rust.

As mentioned earlier, the hot plates can be a good companion in lab works and in daily use as they come at a very reasonable price as compared to other heating appliances. You just have to take good and proper precautions and care for it.