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Posts tagged ‘Centrifuge tubes’

Centrifugation: A widely applied and crucial research technique

In the field of biochemistry, cellular and molecular biology, and even in medicine, centrifugation is one of the most significant and widely applied research techniques. In technical terms, it is a process which involves the use of the centrifugal force for the sedimentation of heterogeneous mixtures with a centrifuge. In layman terms, it is nothing but a process to separate components of a complex mixture or to separate two immiscible liquids by using a centrifuge. This process is an integral part of industry and laboratory settings.

Centrifugation

                   Centrifugation

If we talk about the field of proteomics, centrifugation plays a vital role in the fundamental and necessary process of isolating proteins. It is a known fact that centrifugation is probably the most commonly used and preferred method for separating out all the non-protein material.

Centrifugation Process

              Centrifugation Process

This process has other uses such as:-

Centrifugation Methods

            Centrifugation Method

  • It is used to remove cells or other suspended particles from their surrounding milieu on either a batch or a continuous-flow basis. It also, separates a cell type different from another one.
  • The process isolates viruses and macromolecules, including DNA, RNA, proteins, and lipids or establishes physical parameters of these particles from their usual observed behavior.
  • Centrifugation separates the various sub-cellular organelles including nuclei, mitochondria, chloroplasts, Golgi bodies, lysosomes, peroxisomes, glyoxysomes, plasma membranes, endoplasmic reticulum, polysomic, and ribosomal subunits from the dispersed tissue.

How the separation of components achieved in centrifugation process?

Centrifugation

The separation of the components during centrifugation is achieved by spinning a vessel containing material at a high speed. The imposed centrifugal force pushes heavier materials to the outside of the vessel (in some cases to settle down).

Centrifugation Methods

Centrifugation Methods

Centrifuge Tubes: In the whole process, the most recurring term is the centrifuge tubes. It is a laboratory device that is used for the separation of fluids, gas or liquid, based on density. It works on the principle that when the required samples are spun at a high speed, the resulting force causes the particles to separate based on their density. For example, those particles having a high density will settle down and those with less density will float.

Centrifuge Tubes

              Centrifuge Tubes

This highly useful apparatus is found in most of the laboratories starting from academic to clinical to research laboratories. The device is extremely useful for purification of cells, sub-cellular organelles, viruses, proteins and nucleic acids.

Also, these tubes are available in different types depending upon the rotor design or the intent if use. From the large floor variety to the micro-centrifuge, there are many varieties available for the researcher.

Abdos’ range of Centrifuge Ware

Abdos is a well-known global brand in manufacturing plastic lab-ware products. We started our plastic laboratory division and its operations, back in the year 2009 envisaging a clear vision of delivering quality products that will transform the research world into a better and hassle-free one.

Our broad range of Centrifuge Ware is employed in a variety of centrifugation methods and assay investigations in laboratories. We takes pride in molding these fine lab-products from unadulterated and virgin resins that yield low extractability and leaching while using them.

The products that comprise the Centrifuge Ware are listed below

1)         Micro Centrifuge Tubes, PP

2)         Micro Pestles, PP

3)         Centrifuge Tube Conical Bottom, PP/HDPE

4)         Self Standing Centrifuge Tube

5)         Centrifuge Tube Conical Bottom in Polystyrene

6)         Racked Centrifuge Tube Conical Bottom

7)         Rack for Micro Centrifuge Tubes, RPP

8)         Rack for Micro Centrifuge Tubes, PC

9)         Centrifuge Tube Box, PP

10)      Swirl-01 Micro Centrifuge

Microcentrifuges

     Micro Centrifuge Tubes

Centrifuge Tube Conical Bottom

Centrifuge Tube Conical Bottom

Rack for Micro Centrifuge Tubes

Rack for Micro Centrifuge Tubes

These products act as consumable items in the laboratories while displaying their distinctive characteristics and fine quality during laboratory research processes.

How to Operate the Centrifuge Tubes: Guidelines

The centrifuge tubes are cylinder shaped calibrated glass containers commonly used for the analysis and separation of various materials in the laboratory (centrifugation procedures). They needed to be fit into the centrifuge slots for the experiments.

microcentrifuge tubes

Centrifuge Tubes

These are used to test solid-liquid or immiscible liquid samples by the lab technicians. First, they deposit various substances into the centrifuge tubes and then they secure them into the centrifuge within the slots. For the rotation, the lab technicians spin the tubes at proper speeds until the denser particles in each substance gather at the point farthest from the axis and vice-versa.

Materials used

A wide range of laboratory centrifuge tubes are available in plastic, copolymers, polypropylene and polystyrene.

Sizes

It ranges from micro-centrifuge tubes with caps to larger tubes capable of 50,000 grams in refrigerated centrifuges.

Types

There are different types of centrifuge tubes widely used for different purposes such as:-

Micro Centrifuge Tubes

Microcentrifuge Tubes with Snap Cap

  • Conical Bottom Tubes
  • Microcentrifuge Tubes- Snap Cap
  • Microcentrifuge Tubes- Screw Cap and so on

How to use a centrifuge to avoid mishaps

There are few key guidelines for operating a centrifuge tube in order to prevent damage to the centrifuge and possible serious injuries to the lab doctors or technicians:-

  • Always work on an even surface, don’t use the centrifuge on an uneven surface or a slanted one. The surface should be properly leveled and firm.
  • It is safe to balance the centrifuge tubes in the rotor, say for- if you want to run a centrifuge tube with 20 mL of liquid, it is smart to put another tube with 20 mL of water in the opposing hole on the rotor. And if the liquid has a varying density than water, then the best possible way is to balance the tubes by mass not volume.
  • Never ever, open the lid while the rotor is moving. It can be really dangerous at times. Although, there are many centrifuges that come with a “safety shutoff” if the lid is opened but it only stops the power of the rotor. The rotor will still spin due to its own inertia and thus it is better to be careful at the first place.
  • While the rotation of the centrifuge tubes, a little vibration is normal but excessive wobbling can mean danger. Always, double check whether the tubes are correctly balanced or not. Do not take the risk to continue running a centrifuge that is shaking or wobbling while the rotor is spinning.
  • If you are in a laboratory, it is always preferable to wear a face shield or safety glasses. And if you are working near a centrifuge then it is a must.
  • Do not touch, bump or move the centrifuge while the rotor is still spinning.
  • It is better to clean the rotor and centrifuge well after each use to avoid further disruptions.