Precise & Protective

Posts tagged ‘Micropipette tips’

Key steps to prevent pipetting errors

It is very important to avoid errors during any lab-work or research process. Here are some steps required to prevent pipetting errors during lab-work:-

Pipette tips

                 Micropipette Tips

  • You should clean and check your pipette properly after each use because a contamination-free pipette ensures accuracy. Make it a daily routine. Also, check for any damage prior to beginning work.
  • It is extremely essential to have your pipette serviced after every six months (on an average). The servicing of the pipette includes the complete disassembling of the pipette for re-calibration and replacement of broken parts.
  • Before using the pipettes, you should have a decent knowledge of proper pipetting techniques. Make sure you follow some simple techniques while using a pipette:
  • Start practicing a smooth and slow motion
  • Pre-wet the pipette tip before you start the process
  • Hold the pipette vertically when drawing in liquid and at a 45 degree angle when dispensing in liquid
  • Immerse the tip slightly into the liquid during the aspiration process
  • Touch off the pipette on the sidewall of the container
  • It is very important to use a pipette with a volume selection closest to the volume required for the aspiration or dispensing process. For example, if you need to dispense 15 microliters, a 20 microliter pipette would be an ideal choice.
  • The environmental conditions should be taken into account. You should consider the consistency of temperature, pressure, humidity, etc. before you start using pipettes for any research work. It is important because an inconsistent environment will affect the accuracy of your results as liquids are susceptible to all types of environmental changes. Also, it is safe to allow your pipette to adjust according to the environmental conditions.
  • Always try and keep your pipette safe like never hold your pipette when you’re not using it, never put it down on the lab bench, always keep it on a stand, always store it in an upright position e.tc.
  • It is better to use a new tip every time or whenever you test a new liquid. A tip that is continually used for different tests and substances is susceptible to cross contamination.

These few and simple tips can help you a lot during your research work. Remember, prevention is always better than cure.

Abdos Micro-pipette Tips: Universal and compatible with various other brands of pipettes

Serological Pipettes

             Pasteur Pipette

  • Abdos Tips are manufactured in state of art precision-moulded facility using high purity virgin polypropylene.
  • Micro Tips are DNase, RNase and Pyrogen free.
  • Designed for use in a wide variety of pipetting applications and are compatible with different types of u-Pipettes.
  • Clean and the hydrophobic surface ensures low retention, increased accuracy and reproducibility.
Variable Premium Pipette

          Fixed Premium Pipette

  • More transparency for clear visualization in molecular biology work.
  • High clarity and finer tip orifice makes the tip more suitable for molecular biology and precise applications.
  • Perfect airtight grip on pipette by inner radial rings for accurate pipetting.
  • The orifice is precisely centered for directional accuracy.
  • Can be autoclaved

For more information on code, capacity or compatibility of the Abdos micro-pipettes, log on to http://www.abdoslabware.com/micro-tips.html

How to use the Micropipette Tips

Pipettes are a common sight in any chemistry, biology or a physics laboratory. Popularly known as pipette, pipettor, chemical dropper or a pipet, it is a common laboratory tool to transfer a measured volume of liquid. They are available in different designs for different purposes.

High Quality Micropipette Tips

Micropipette Tips Sizes

Micropipettes are those dispensing between 1 and 1000 μl and in contrast, macropipettes dispense greater volumes. Depending upon the sizes, capacity and style, pipettes is of following types:

•    Air displacement micropipettes
•    Positive displacement pipette
•    Volumetric pipettes
•    Graduated pipettes
•    Pasteur pipette
•    Transfer pipettes
•    Van Slyke pipette
•    Ostwald–Folin pipette
•    Glass micropipette
•    Extremely low volume pipettes
•    Microfluidic pipette

Micro Pipettes

Laboratory Micropipettes

But before using a pipette, it is extremely necessary that you master their use in order to be successful in the lab experiments.

Parts of Micropipette

a)    Plunger button
b)    Tip ejector button
c)    Volume adjustment dial
d)    Digital volume indicator
e)    Shaft
f)    Attachment point for a disposable tip

Sizes of micropipette

Basically, micropipettes come in three different sizes of varying capacity. They are P20, P200 and P1000. The sizes are noted on the top of the plunger button (as mentioned earlier, part a)

Pipette Tips

You should always remember that liquids are never drawn directly into the shaft of the pipette. For this, a disposable plastic tips are attached to the shaft and for adjusting the volume of the liquid, there is a black volume adjustment dial near the top of the micropipette.

There are two sizes of tips. The larger blue tips are used for the P1000 and the smaller clear tips are used for the P20 and P200. You should also take care of not contaminating the tips, for example: – close the box of the tips (racked in), after the use so that airborne contamination can be prevented.

How to insert a tip

Micropipette tips uses

Micropipette tips function

Before inserting a tip, you should keep some essential points in mind as given below:-
•    Select the correct size tips.
•    Open the box without touching the tips with your hands.
•    Insert the micropipette shaft into the tip and press down firmly. This will attach the tip to the shaft.
•    Remove the micropipette, with the tip attached.
•    Close the box without touching the tips with your hands
•    Remember, it is better to handle something carefully rather than doing it like a defaulter.